Allogeneic hemopoietic stem cell transplantation using mouse spleen cells fractionated by lectins: in vitro study of cell fractions.

Transplantation and Homing of Mouse Embryonic Stem Cells Treated with Erythropoietin in Spleen and Liver of irradiated mice

Background: The present study was designed to evaluate the homing potential of mouse embryonic stem cells (ESC) treated with erythropoietin (EPO) in hematopoietic organs such as spleen and liver after transplantation using morphological and immuno-histochemical techniques. Methods: Day-four embryoid body (EB)-derived cells were dissociated and re-plated in medium in the presence and absence of ...

Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method

Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Mouse nidovirus LDV infection alleviates graft versus host disease and induces type I IFN‐dependent inhibition of dendritic cells and allo‐responsive T cells

INTRODUCTION Viruses have developed multiple mechanisms to alter immune reactions. In 1969, it was reported that lactate dehydrogenase-elevating virus (LDV), a single stranded positive sense mouse nidovirus, delays skin allograft rejection and inhibits spleen alterations in graft versus host disease (GVHD). As the underlying mechanisms have remained unresolved and given the need for new therapi...

Fetal microchimerism in mouse caerulein-induced pancreatitis model

Objective(s): Fetal microchimerism is the persistence of allogeneic cell population that transfer from the fetus to the mother. The aim of this study was to evaluate the presence of fetal microchimerism in the pancreas of the mouse with acute pancreatitis (AP).Materials and Methods: In this experimental study, female wild-type mice were mated with male EGFP+. AP model was obtained by injection ...